Plasticizers excreted in urine: indication of autologous blood transfusion in sports (2023)

Monfort, N., Ventura, R., Platen, P., Hinrichs, T., Brixius, K., Schänzer, W., Thevis, M., Geyer, H., & Segura, J. (2012). Plasticizers excreted in urine: indication of autologous blood transfusion in sports. Transfusion, 52(3), 647-657. https://doi.org/10.1111/j.1537-2995.2011.03331.x

Monfort, Núria ; Ventura, Rosa ; Platen, Petra et al. / Plasticizers excreted in urine : indication of autologous blood transfusion in sports. in: Transfusion. 2012 ; Jahrgang 52, Nr. 3. S. 647-657.

@article{32ff9b9cc20146bbb1b8588b8354386b,

title = "Plasticizers excreted in urine: indication of autologous blood transfusion in sports",

abstract = "BACKGROUND: Misuse of autologous blood transfusions in sports remains undetectable. The metabolites of the plasticizer di-(2-ethylhexyl)phthalate (DEHP) were recently proposed as markers of blood transfusion, based on high urinary concentrations of these compounds observed in patients subjected to blood transfusion. This study evaluates DEHP metabolites in urine for detecting autologous blood transfusion.STUDY DESIGN AND METHODS: One blood bag was drawn from moderately trained subjects and the red blood cells (RBCs) were reinfused after different storage periods. Group 1 (12 subjects) was reinfused after 14 days, and Group 2 (13 subjects), after 28 days of storage. Urine samples were collected before and after reinfusion for determination of the concentrations of three DEHP metabolites, mono-(2-ethylhexyl)phthalate, mono-(2-ethyl-5-hydroxyhexyl)phthalate, and mono-(2-ethyl-5-oxohexyl)phthalate.RESULTS: Concentrations of DEHP metabolites on the days before reinfusion were in agreement with those described after common environmental exposure. A few hours after the reinfusion a significant increase was observed for all metabolites in all volunteers. Concentrations 1 day later were still higher (p < 0.05) than before reinfusion. Variations in urine dilution supported normalization by specific gravity. Concentrations of DEHP metabolites tended to be higher after longer storage times of RBCs.CONCLUSION: Autologous transfusion with RBCs stored in plastic bags provokes an acute increase in the urinary concentrations of DEHP metabolites, allowing the detection of this doping malpractice. The window of detection is approximately 2 days. The method might be applied to urine samples submitted for antidoping testing.",

keywords = "Adult, Blood Transfusion, Autologous, Diethylhexyl Phthalate, Doping in Sports, Female, Humans, Male, Phthalic Acids, Plasticizers, Specific Gravity, Urine, Young Adult",

author = "N{\'u}ria Monfort and Rosa Ventura and Petra Platen and Timo Hinrichs and Klara Brixius and Wilhelm Sch{\"a}nzer and Mario Thevis and Hans Geyer and Jordi Segura",

note = "{\textcopyright} 2011 American Association of Blood Banks.",

year = "2012",

month = mar,

day = "1",

doi = "10.1111/j.1537-2995.2011.03331.x",

language = "English",

volume = "52",

pages = "647--657",

journal = "Transfusion",

issn = "1537-2995",

publisher = "Wiley-Blackwell",

number = "3",

}

Monfort, N, Ventura, R, Platen, P, Hinrichs, T, Brixius, K, Schänzer, W, Thevis, M, Geyer, H & Segura, J 2012, 'Plasticizers excreted in urine: indication of autologous blood transfusion in sports', Transfusion, Jg. 52, Nr. 3, S. 647-657. https://doi.org/10.1111/j.1537-2995.2011.03331.x

Plasticizers excreted in urine : indication of autologous blood transfusion in sports. / Monfort, Núria; Ventura, Rosa; Platen, Petra et al.

in: Transfusion, Jahrgang 52, Nr. 3, 01.03.2012, S. 647-657.

Publikation: Beitrag in Fachzeitschrift › Zeitschriftenaufsätze › Forschung

TY - JOUR

T1 - Plasticizers excreted in urine

T2 - indication of autologous blood transfusion in sports

AU - Monfort, Núria

AU - Ventura, Rosa

AU - Platen, Petra

AU - Hinrichs, Timo

AU - Brixius, Klara

AU - Schänzer, Wilhelm

AU - Thevis, Mario

AU - Geyer, Hans

AU - Segura, Jordi

N1 - © 2011 American Association of Blood Banks.

PY - 2012/3/1

Y1 - 2012/3/1

N2 - BACKGROUND: Misuse of autologous blood transfusions in sports remains undetectable. The metabolites of the plasticizer di-(2-ethylhexyl)phthalate (DEHP) were recently proposed as markers of blood transfusion, based on high urinary concentrations of these compounds observed in patients subjected to blood transfusion. This study evaluates DEHP metabolites in urine for detecting autologous blood transfusion.STUDY DESIGN AND METHODS: One blood bag was drawn from moderately trained subjects and the red blood cells (RBCs) were reinfused after different storage periods. Group 1 (12 subjects) was reinfused after 14 days, and Group 2 (13 subjects), after 28 days of storage. Urine samples were collected before and after reinfusion for determination of the concentrations of three DEHP metabolites, mono-(2-ethylhexyl)phthalate, mono-(2-ethyl-5-hydroxyhexyl)phthalate, and mono-(2-ethyl-5-oxohexyl)phthalate.RESULTS: Concentrations of DEHP metabolites on the days before reinfusion were in agreement with those described after common environmental exposure. A few hours after the reinfusion a significant increase was observed for all metabolites in all volunteers. Concentrations 1 day later were still higher (p < 0.05) than before reinfusion. Variations in urine dilution supported normalization by specific gravity. Concentrations of DEHP metabolites tended to be higher after longer storage times of RBCs.CONCLUSION: Autologous transfusion with RBCs stored in plastic bags provokes an acute increase in the urinary concentrations of DEHP metabolites, allowing the detection of this doping malpractice. The window of detection is approximately 2 days. The method might be applied to urine samples submitted for antidoping testing.

AB - BACKGROUND: Misuse of autologous blood transfusions in sports remains undetectable. The metabolites of the plasticizer di-(2-ethylhexyl)phthalate (DEHP) were recently proposed as markers of blood transfusion, based on high urinary concentrations of these compounds observed in patients subjected to blood transfusion. This study evaluates DEHP metabolites in urine for detecting autologous blood transfusion.STUDY DESIGN AND METHODS: One blood bag was drawn from moderately trained subjects and the red blood cells (RBCs) were reinfused after different storage periods. Group 1 (12 subjects) was reinfused after 14 days, and Group 2 (13 subjects), after 28 days of storage. Urine samples were collected before and after reinfusion for determination of the concentrations of three DEHP metabolites, mono-(2-ethylhexyl)phthalate, mono-(2-ethyl-5-hydroxyhexyl)phthalate, and mono-(2-ethyl-5-oxohexyl)phthalate.RESULTS: Concentrations of DEHP metabolites on the days before reinfusion were in agreement with those described after common environmental exposure. A few hours after the reinfusion a significant increase was observed for all metabolites in all volunteers. Concentrations 1 day later were still higher (p < 0.05) than before reinfusion. Variations in urine dilution supported normalization by specific gravity. Concentrations of DEHP metabolites tended to be higher after longer storage times of RBCs.CONCLUSION: Autologous transfusion with RBCs stored in plastic bags provokes an acute increase in the urinary concentrations of DEHP metabolites, allowing the detection of this doping malpractice. The window of detection is approximately 2 days. The method might be applied to urine samples submitted for antidoping testing.

KW - Adult

KW - Blood Transfusion, Autologous

KW - Diethylhexyl Phthalate

KW - Doping in Sports

KW - Female

KW - Humans

KW - Male

KW - Phthalic Acids

KW - Plasticizers

KW - Specific Gravity

KW - Urine

KW - Young Adult

U2 - 10.1111/j.1537-2995.2011.03331.x

DO - 10.1111/j.1537-2995.2011.03331.x

M3 - Journal articles

C2 - 21895677

SN - 1537-2995

SN - 0041-1132

VL - 52

SP - 647

EP - 657

JO - Transfusion

JF - Transfusion

IS - 3

ER -

Monfort N, Ventura R, Platen P, Hinrichs T, Brixius K, Schänzer W et al. Plasticizers excreted in urine: indication of autologous blood transfusion in sports. Transfusion. 2012 Mär 1;52(3):647-657. doi: 10.1111/j.1537-2995.2011.03331.x